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A new validatedmethod for determination of tembotrione and its metabolite residues in orange fruit

Tentu.Nageswara Rao, D.Sreenivasulu, S.N.V.S.Murthy, K.Raghu Babu

A simple and inexpensive method was developed using solid-phase extraction, together with high performance liquid chromatographic method with UV detection for determination of tembotrione and its metabolite (tembotrione – dihydroxy (AE1417268)) residues. The evaluated parameters include the extracts by florisil packed column using hexane: ethyl acetate solventmixture (2:3),methanol and acetonitrile solvents. Themethod was validated using fruit samples spiked with tembotrione and its metabolite (tembotrione – dihydroxy) at different fortification levels (0.03 and 0.3 µg/g). Average recoveries (using each concentration six replicates) ranged 84-94%, with relative standard deviations less than 2%, calibration solutions concentration in the range 0.03-10.0 µg/mL and limit of detection (LOD) and limit of quantification (LOQ) were 0.01µg/g and 0.03µg/g respectively. Finally the fruit residue samples were analyzed byHPLC.