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A SIMPLE HPLC METHOD FOR THE ESTIMATION OF ANDROGRAPHOLIDE

Yedugani Lingam, Gajanan Patange, Atish Jayaprakas Salunke

A simple, rapid, selective and quantitative HPLC method has been developed for the determination of Andrographolide. Mobile phase was Water: Acetonitrile (60: 40), Flow rate 1.0 ml/min. Retention time of pure Andrographolide was 5.038 minutes. The content of andrographolide in crude (BNPL/AD/003) was 30% and reasonable pure sample (BNPL/AD/006) purity is 82% respectively. The developed method was found to be simple, robust, rugged, and economic for routine use in the herbal drug industry.