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A validated chiral HPLC method for the enantiomeric separation of ramipril
G.Ramulu, K.Rama Seshaiah, Y.Ravindra Kumar, K.Vyas, K.Mukkanti, M.V.Suryanarayana
An isocratic chiralHPLCmethodwas developed for the separation ofRamipril enantiomers. The mobile phase consists of n-hexane and 2-Propanol in the ratio of 900: 100(v/v)with 0.2ml volumes of Tri fluoro acetic acid and 0.1ml volume of Di ethyl amine. Thismethod is capable of detecting the Ramipril RRRRR-isomer up to 0.3µg/ml. The method was validated interms of Precision, Linearity,Accuracy, and Limit of detection and Limit ofQuantification.
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