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Advanced Chromatographic Techniques for the Analysis of Sugars Extracted from Sweet Lemon Peels

C. S. Chidan Kumar, R. Mythily and S. Chandraju

A rapid, sensitive extraction method was developed using the mixture MethanolDichloromethane - Water (MDW) (0.3 : 4 : 1, v) and MeOH-H2O phase was assayed for sugar analysis. High-performance liquid chromatography (HPLC) with Evaporative Light Scattering Detector (ELSD) coupled to electro spray ionization mass spectrometric (ESI-MS) detection in the positive ion mode gave MS and MSn fragmentation data, which were employed for their structural characterization. The various standard sugars were spotted using the solvent system n-butanol-acetone-pyridine-water (10 : 10 : 5 : 5, v) in the cellulose layer for TLC analysis, which indicated the presence of galactose, glucose, arabinose and Xylose. This is the first assay of the sugar profile of the sweet lemon peels, which can be further developed for characterization and evaluation of their quality with regards to their sugar composition