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An HPLC method for the determination of busulfan in biological samples

M.A.Navarro Moreno, A.Moreno Galvez, L.Moreno Galvez

An HPLC analytical method for assessing plasma busulfan rat has been developed. Thismethod uses 1,5-Bis (metansulfoniloxy) pentane, as internal standard, dimethylformamide (DMF) as solvent, Tri Butyl n Phosphine as an antioxidant and 8Mercaptoquinoline (Q) as a reagent.We have evaluated the accuracy, reproducibility and speed, with satisfactory results.