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Bioanalytical Method Development and Validation for the Simultaneous Estimation of Indomethacin and Omeprazole in Rabbit Plasma by Using HPLC

G. Raveendra Babu, A. Lakshmana Rao and J. Venkateswara Rao

A novel approach was used to develop and validate a bioanalytical RP-HPLC method for the simultaneous estimation of Indomethacin and Omeprazole in rabbit plasma using Rabeprazole as internal standard. Evaluation of the content of drugs were done by employing a mixture of phosphate buffer (pH 4.6) and acetonitrile (65:35, v/v) as the mobile phase and measure the absorbance at 260 nm for Indomethacin and Omeprazole. Retention time was established to be 2.014 min for Rabeprazole, 3.310 min for Indomethacin and 5.479 min for Omeprazole. The results have shown that the analytical technique furnished here establishes acceptable accuracy and precision, shorter and easy sample preparation, reduced the complications for equipment on satisfactory analysis time.