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Chemical Constituents of Ethanol Extract and Free Radical Scavenging Activity of Vitex Trifolia Linn

J. S. Jangwan, R. P. Aquino, T. Mencherini, P. Picerno and R. Singh

An Iridoid agnuside has been isolated from ethanolic extract of Vitex trifolia leaves. The compound was characterized using spectroscopic methods including 1H NMR, 13C NMR, ESIMS and 2D-NMR (HSQC, HMBC, COSY) experiments and confirmed by comparison of NMR data with those from the literature. DPPH free radical scavenging activity for ethanol extract of leaves with EC50 value 0.478 mg/mL was found more effective than chloroform extract with EC50 value 0.602 mg/mL and n-hexane extract with EC50 value 0.794 mg/mL. NO free radical scavenging activity for ethanol extract of leaves with EC50 value 0.524 mg/mL was found more effective than chloroform extract with EC50 value 0.660 mg/mL and n-hexane extract with EC50 value 0.851 mg/mL