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Colorimetric determination of acetazolamide in pharmaceutical preparation by simple diazotization method

B.Ihssane, M.Charrouf, A.Abourriche, T.Saffaj, Y.Abboud, S.Maouffoud, A.Bennamara

Acolorimetricmethod for determination of acetazolamide is described. The method is based on the formation of red coloured azo product by diazotization of acetazolamide molecule followed by a coupling reaction with N-(1-naphthyl) ethylenediamine dihydrochlorhydrate in acidmedium. Absorbance of the resulting red azo product is measured at 493nm and is conforms to Beer’s law over the range 10-200gml-1. The optimumreaction conditions were evaluated and common excipients used as additives in pharmaceutical preparations do not interfere in the proposed method. The method is simple, sensitive and rapid also is successfully employed for the determination of acetazolamide in pharmaceutical preparations.