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Comparative extraction methods, phytochemical constituents, fluorescence analysis and HPLC validation of rosmarinic acid content in Mentha Piperita, Mentha longifolia and Osimum basilicum

Aveen Nozad Adham

The present investigation is aimed to compare the percentage value of Mentha Piperita, Mentha longifolia and Osimum basilicum extracts by using different solvent and extraction methods, phytochemical screening of constituents present in dry leaves, also to study fluorescent characteristics of leaves powder which are responsible for the medicinal properties of the plant using various standard tests and HPLC analysis of rosmarinic acid content in three labiatae species. The highest percentage extract was yielded fromMentha longifolia (18.5%) by usingmacerationmethods and ethanol 80% solvent. Phytochemical analysis revealed the presence of alkaloids, saponins, phenols, flavonoids, terpenoids, cardioactive glycosides, tannins, carbohydrates in dry leaf extracts. Presence of alkaloids and phenolic acid was determined in high quantity in Mentha longifolia, while saponins present in high quantity in Mentha Piperita and flavonoids in Osimum basilicum. The fluorescent characteristics of leaves powder with various chemical reagents were noted under visible and UV light. HPLC analysis of dry leaf extracts revealed presence of rosmarinic acid among the investigated plants and in higher percentage in Osimum basilicum (0.306%).