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Determination of cilnidipine from pharmaceutical formulation by high performance thin layer chromatographic method

H.S.Karmalkar, V.V.Vaidya, N.A.Gomes, M.P.Choukekar, M.B.Kekare

A suitable method was developed and validated for the determination of cilnidipine byHPTLC frompharmaceutical formulation usingNifedipine as an internal standard. The analyte and internal standard were resolved on silica gel 60F254 HPTLC plates (Merck) by using a two component mobile phase system comprising toluene-ethyl acetate, 6.0:4.0 (v/v) with a chamber saturation of 8mins. The plate was developed up to 8cm and air-dried. The plate then was scanned and quantified at 238nm. The linear dynamic range of cilnidipine was found to be 5g mL-1 to 45g mL-1. The limit of detection and limit of quantification for cilnidipine was found to be 3g mL-1 and 5g mL-1 respectively. The proposed method is accurate, precise and rapid for the determination of cilnidipine.