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Determination Of Protein In Human Serum By Acid Brown SR Resonance Light Scattering

Baosheng Liu, Zhichao Liu, Jing Gao

A quantitative method was developed for the determination of proteins in aqueous solution. Under optimal conditions, the linear ranges of calibration curves for the determination of bovine serum albumin (BSA), human serum albumin (HSA) and egg albumin (Alb) were 0- 1.13 μg ml-1, 0-1.17μg ml-1 and 0-1.13μg ml-1, respectively. The detection limits were 15.74 ng ml-1 for BSA, 23.34 ng ml-1 for HSA and 20.75 ng ml-1 for Alb. The method was applied to analysis of total protein in human serum samples collected from the hospital and the results were in good agreement with those reported by a local hospital.