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Development and validation of RP-HPLC method for estimation of felodipine in rat plasma

Minal Raghunath Narkhede, Bhanudas Shankar Kuchekar, Jitendra Yadav Nehete

An attempt has been made to develop a simple, precise, sensitive, reproducible and rapid for estimation of felodipine in rat plasma by reverse phase HPLC. Chromatographic separation was achieved on a Spherisorb (250mmï‚´4.6mm, 5 ìm) ODS column using mobile phase (methanol and water) in the ratio 80:20%v/v at a flowrate of 0.9ml/min. Thewavelength of detection was 260 nm. The proposed chromatographic method gave good resolution of the drug in plasma. Felodipine eluted at retention time of 9.94 min. The linearity of themethod was established in the range 50 ng - 150 ng/ mlwith a correlation coefficient of 0.9943 The limit of detection of felodipine was 25 ng/ml and limit of quantification was 50 ng/ml. Excellent precision was evident with a coefficient of variance less than 15%. The developed HPLC method was found to be linear, precise, accurate and reproducible indicatingmethod may be used for the quantitative estimation of felodipine in blood samples.