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Development and Validation of RP-HPLC Method for the Estimation of Antiretroviral Drugs in Tablet Dosage Forms

K. Parameshwar Rao

Reversed phase liquid chromatographic method has been developed and subsequently validated for the determination of drugs in tablet formulations. Standard stock solutions of different drugs were prepared by transferring accurately weighed drug and dissolving in a solution suitably. This solution was sonicated for 20 min to achieve complete dissolution and made up to the mark with mobile phase. From the standard stock solution different concentrations of working standard solutions of drugs were prepared with the same mobile phase. The developed method offers several advantages in terms of simplicity in mobile phase, mode of elution, easy sample preparation steps and comparative short run time which makes the method specific and reliable for its intended use in routine analysis determination of drug in tablet dosage forms.