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Development and validation of UV- spectrophotometric method for the determination of doxazosin mesylate in pharmaceutical formulations

N.V.S.Naidu, K.Babu Naidu, P.Suguna

A simple, sensitive, selective rapid spectrophotometric method was developed for the determination of post synaptic á-1 Adrino receptor antagonist Doxazosin mesylate in pure form and pharmaceutical formulations based on the oxidation of Fe3+ion in presence of drug and followed by orange red colored complex formation with 1,10- Phenantrolline reagent at PH 5 which are extractable at 510 nm. BeerÂ’s law is obeyed in the concentration range 10-60 µg /ml. The developed method was applied directly and easily for the analysis of the Pharmaceutical formulations. R.S.D was found to be 0.53180 and recovery 99.32% respectively. The method was completely validated and proven to be rugged. The interferences of the other ingredients and excipients were not observed. The repeatability and the performance of the proved method were established by point and internal hypothesis and through recovery studies.