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Diversity Analysis by Random Primer Amplification of Genomic DNA of Mulberry (Morus spp.) Germplasm Collection from Traditional Sericulture States of India

Guru Prasad and Dandin SB

Genetic diversity assessment and cluster analysis were carried out among thirty-sixmulberry germplasmassembled fromdifferent parts of the country by genomicDNAamplification of 22 informative randomprimers. PCR amplification resulted in 226 markers out of that 208 markers were polymorphic (92.04%) and as many as 11 primers were showed 100% polymorphism. Dice dissimilaritywas calculated using DNA marker scores and the marker index among the pairs of germplasm ranged from 0.081 to 0.371. Meergunj and Bisnupur-4 recorded the least dissimilarity where as Rajouri and Nagaland Local were most divergent. The mean dissimilarity among the germplasmcollection was 0.226. UPGMAclustering topology showed 6 distinct groups, which mainly comprised of accessions from the similar geographical origin with exception of Kollegal and Kajali. A sub-set of germplasm with a sample intensity of about 10% of the original collection was identified which comprised ofAssamjati, Ficus 2x, Berhampur-6 and Sujanpur x Philippines based on the active score (363) and Shanon diversityindex( 85.06) usingmaximization strategyimplemented through MSTRAT algorithm.