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Efficient extraction of flavonoids of sea buckthorn berries

Merja Heinaaho, Riitta Julkunen-Tiitto

Six different extractionmethodswere tested to establish an efficientmethod for extracting flavonoids from sea buckthorn (H. rhamnoides L. ssp. rhamnoides) berries. Twelve flavonoids were studied and HPLC was used to quantitate recovery. The content of total HPLC flavonoids was higher in acetone/petroleum ether extracts compared to other extraction methods. Acetone/petroleum ether extraction optimised recovery of quercetin derivative (1), isorhamnetin 3,7-diglucoside, isorhamnetin rhamnodiglucoside, isorhamnetin 3-glucoside 7-rhamnoside, isorhamnetin-glucoside (3), quercetin derivative (3) and dicoumaroyl isoquercitrin compared to the other extraction methods. Acetone/petroleum ether, hexane, methanol and ethanol as extraction solvents gave similar concentration for five other compounds, while propanol/hexane and chloroform/methanol were not found to be useful extraction solvents due to very lowextraction efficiency. These results indicate that the extraction of flavonoid compounds of sea buckthorn berries is maximized using acetone/petroleum ether extraction method.