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Estimation and validation of metformin by UV spectroscopy

Vijay Vikram Singh,Amit Kumar, G.T.Kulkarni

A simple highly sensitive spectrophotometric method was developed for the quantification of Metformin hydrochloride (N, N-dimethylimidodicarbonimidic diamide). U.V.Spectrophotometric estimation ofMetformin hydrochloride in bulk and in formulation using 0.1M Sodium hydroxide (NaOH) solution as solvent. The absorptionmaximawasmeasured at 233nm. The linearity was found in the concentration range 5-25mcg/ml. The proposed method can be utilized as a stability indicating assay. Under the described conditions the proposed method is linear over the concentration range of 5-25mcg/ml and the coefficient of determinationwere >0.999with a relative standard deviation of 0.702105%. The average recovery of the target compound is 98.62%with a limit of quantification (LOQ) of 158.13mcg/ ml and the limit of detection (LOD) 52.183mcg/ml.