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Estimation of eflornithine hydrochloride by UV spectroscopy

Amit Kumar, S.P.Venkatesh Prasad, S.Mohan, Aklavya Kumar Singh

A simple highly sensitive spectrophotometric method was developed for the quantification of Eflornithine hydrochloride (2-difluoromethyl-DL-ornithine; DFMO). U.V. Spectrophotometric estimation of Eflornithine hydrochloride (DFMO) in bulk and in LargeVolume Parental (L.V.P.) formulation using 0.1MSodiumhydroxide (NaOH) solution as solvent. The absorption maximawasmeasured at 215.0 nm. The linearitywas found in the concentration range 50.0-400.0 mcg/ml. The proposed method can be utilized as a stability indicating assay. Under the described conditions the proposed method is linear over the concentration range of 50-400 mcg/ml and the coefficient of determination were >0.999 with a relative standard deviation of 0.001 %. The average recovery of the target compound is 99.99%with a limit of quantification (LOQ) of 8.41mcg/ml and the limit of detection (LOD) 2.77mcg/ml.