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Estimation of hydroxycitric acid (-)-hca in garcinia indica choisy by HPLC method

Mote Satish, Seema Misra-Thakur

Asimple, fast and precise reverse phase High performance liquid chromatographic isocratic method has been developed. The aim of the study was to develop a High performance liquid chromatography (HPLC) assaymethod for accurate determination of (-)-HCA in Garcinia Indica choisy. (-)- Hydroxycitric acid is the principal acid of fruit rinds of Garcinia cambogia and Garcinia Indica. The experimental procedure involved Diode Array Detector (at 210nm), Mobile phase (0.01M Potassium dihydrogen orthophosphate in purified water and adjust the pH to 2.80 with orthophosphoric acid,with the flowrate 1.0ml/min) and InertsilODS 3V(250 4.6mm, 5size) column. A calibration curve of mean peak area vs. concentration showed good linearity.The linearity range selected for the experiment is from50%to 150% of the actual concentration level i.e. 50ppm to 150ppm.The active ingredient , Hydroxy citric acid(-)-), is weight loss promoter extracted from the rind of the fruit. The fruit rinds of Garcinia Indica contain 8%(-)-HCA.