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GC-MS and 13C NMR study of epicuticular waxes of olive fruit(Olea europaea)cv. Dritta

Giovanna Vlahov, Giuseppe Rinaldi, Paolo Del Re, Angela Alessia Giuliani

The chloroform-soluble waxes of the olive fruit(Olea europaea) of cv. Dritta were separated by thin layer chromatography(TLC) and analyzed by gas liquid chromatography(GLC) and gas liquid chromatography-mass spectrometry(GC-MS). The classes of different compounds include apolar molecules(n-alkanes, aldehydes and benzyl esters), long chain aliphatic alcohols(C18-C30), triterpenic dialcohol(erythrodiol) and triterpenic acids(oleanolic andmaslinic acid).Afurther study on the crude waxeswas carried out by using high-resolution 13C nuclear magnetic resonance spectroscopy(13C NMR).