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HPTLC Finger Prints Of Ephedra Gerardiana Wall And Sida Acuta Burm

Rasheed-uz Zafar, Sayeed Ahmad

High performance thin layer chromatography becomes a very important tool for identification and quantification of marker compounds as well as for checking purity and identity of the drugs. The HPTLC finger print profile of methanolic extracts of Ephedra gerardiana and Sida acuta was established for detection of marker compounds. The chromatogram was developed by using solvent system toluene: chloroform: ethanol (28.5: 57: 14.5) and scanned at 366 nm wavelength. Ephedrine (Rf 0.25) was found present in both the samples a new simple and rapid method for quantitative estimation of ephedrine in Ephedra gerardiana and Sida acuta was developed using standard ephedrine. The content of ephedrine was found 0.88 and 0.86 % w/w in Ephedra gerardiana stem and Sida acuta leaves respectively.