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Induction of alpha and Mu-class of rat liver cytosolic glutathione S-transferase upon chronic alcohol administration

Rukhsana Sultana, Arumugam Padmini, O.H.Setty, Phanithi Prakash Babu

The present study reports the effect of alcohol administration on the expression of alpha andmu-class of cytosolicGST in rat liver.Onemonth old Wistar strain rats were divided into two groups i.e., control, and alcohol group. Control group received basal diet and water and alcohol group received basal diet and 10%alcohol for 2months. Liver was dissected and processed for various biochemical analysis like Western blots, Northern blot, HPLC, IHC and LC-ESI-MS. In alcohol fed group, rGSTM2 protein level was found to be upregulated as evidenced by western blot and HPLC analysis. Increased rGSTA1 and rGSTM2 immunoreactivity in perivenous area was observed compared to controls. In addition, LC-ESIMS analysis showed an 8 fold increase in rGSTM2 subunit. Differential induction and modification of alpha and mu class rGST subunit and its impaired function may contribute to the progression of alcohol-induced liver damage.