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Interleukin -10 (-597), (-824) and (-1082) polymorphisms in the development of gestational diabetes mellitus

Shabnam Montazeri

Introduction: The aim of this study is to investigate the association between single nucleotiode polymorphisms (SNP) in the human promoter of the interleukin-10 (IL-10) gene at positions -1082, -824 and -597 with the development of GDM. Methods: DNAfrompositions -1082, -824 and -597 in the promoter region of human IL-10 gene was amplified by PCR. Restriction fragment length polymorphism (RFLP) was performed on the purified PCR product to determine genotype and allelic frequencies of the three SNP. Plasma levels of IL-10 in different stages of pregnancy as well as six-weeks postpartum were quantified using enzyme linked immunosorbent assay (ELISA). Results: The results showed that therewas a significant (p<0.05) difference in the genotype and allelic frequency of SNP at position -597 between the control and GDM subjects recruited in this study. This was not observed with the other two SNP (-1082 and -824) studied. However, there were no significant differences in plasma IL-10 levels between the two groups. Conclusion: The SNP at position -597 in the promoter of the human IL-10 gene may be associated with development of GDM and it has a potential to be further investigated as a potential tool to be used as a predictive factor using a larger scale trial.