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Investigation of the Reaction of Peroxynitrite with Myoglobin for Meat Extract Samples using Cobalt Phthalocyanine-Modified Screen-Printed Carbon Electrodes and a Flow Injection Analysis System

Falcon Jason

The effects of Reactive Oxygen/Nitrogen Species (ROS/RNS) on cellular responses to stress, cell growth, cell death, cancer, ageing, and male infertility are significant. Monitoring the quality and freshness of raw meat is also crucial for both the food business and consumers. A variety of indicators, including as discoloration, rancidity, and taste changes, might point to meat that has been altered. The scavenging activity of myoglobin against RNS (like Peroxynitrite, PON) is one mechanism of modification. Using Cobalt Phthalocyanine (CoPc) as a straightforward, affordable, highly thermally stable, and biomimetic catalyst, this paper describes the development of an electrochemical PON sensor and the use of this sensor, which is based on Screen-Printed Carbon Electrodes (SPCEs), to analyse meat extract samples using Flow Injection Analysis (FIA). Peroxynitrite reduction is mediated by CoPc. SEM, FTIR, and cyclic voltametry were used to characterize the surface of the modified electrode. Both UV-Vis and chronoamperometry were used to investigate the interaction of PON with myoglobin (at 0.1 V, using the FIA system). The electrode was calibrated using these equations: Ired (n_A)=6.313 CPON (M)+17.469; (R=0.9938). The linear range was 3 M-180 M, and the computed LOD was 2.37 M. Pre-treatment (electro-reduction of the CoPc deposited layer, at 0.3 V, for 60 s) can further enhance the performance of the electrode. In a very selective, sensitive, and repeatable manner, this might assist us in monitoring and quantifying how much PON was destroyed and when meat extracts are treated with various PON concentrations.