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Liquid chromatography-tandem mass spectrometric method for determination of puerarin from Pueraria tuberosa

Harshad P.Mhaske, Vikas V.Vaidya, Shveta H.Mhaske, Pramod N.Pabrekar, Maharudra B.Kekare

Pueraria tuberosa is a commonly used Chinese herbal medicine, which contains a series of isoflavones as its chief pharmacologically active constituents. Using puerarin asmarker, a rapid, reproducible and efficient liquid chromatography-tandemmass spectrometry (LC-MS/MS)method has been developed to quantify puerarin from Pueraria tuberosa. The analyte and Internal Standard (IS) were chromatographed on a Hypurity C18 (50mm4.6mm i.d., 5 particle size) column using 10L injection volume with a run time of 2 min. The precursor and product ion of analyte and IS were monitored on a triple quadrapole mass spectrometer, operating in the multiple reaction monitoringmode (MRM) with positive polarity. The proposed method was validated over the range of 25g/ml to 750g/ml. The proposed LC-MS/MS method was validated for linearity, accuracy, precision and limit of quantitation.