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NOVEL METHOD OF BIOCONVERSION OF WASTE GLYCEROL ACQUIRED DURING BIODIESEL PRODUCTION

Mandar Karve, Jay J.Patel, V.K.Sinha, Nirmal K.Patel

The bioconversion of glycerol was carried out using Lactobacillus strain. Lactobacillus was grown on nutrient broth. The pre-culture was added to MRS (De Man, Rogosa and Sharpe) broth medium where glycerol was used as carbon source to acquire 1,3-propanediol (1,3-PDO). Bioconversion of glycerol was carried out at 200C, 250C, 300C, 350C and 400C, using Lactobacillus strain. The atmospheric pressure and temperature were maintained for 1, 2, 3 and 4days. Optimum result was obtained at 350C and 3rd day where 0.59mole of 1,3-PDO produced per mole of glycerol. Resulted 1,3-PDO was characterized by Gas chromatography (GC), Fourier Transform Infrared Spectroscopy (FTIR) and Proton Nuclear Magnetic Resonance (1H NMR) techniques.