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Phytochemical, Brine Shrimp Lethality Assay, Antibacterial and Antioxidant Activity Screening of Nypafruticans (Nipa) Leaf Extracts

Charlie A. Lavilla Jr and Marvelous Grace L. Villazorda

The present study was designed to evaluate the phytochemicals, cytotoxicity and antioxidant activities of Nypafruticans extracts. Phytochemical screening revealed the presence of flavonoids (23.61%), alkaloids (2.64%), phlobatannins, saponins, and steroids. The ethyl acetate extract at 1000 ppm showed bioactivity (92% mortality) against naupliiArtemiasalina. The extracts showed no inhibition against B. subtilis and E. coli. Furthermore, the antioxidant activity of the extracts were tested by its ability to reduce Mo (VI) to Mo (V) through subsequent formation of green phosphate Mo(V)-complex at acidic pH and positive results were observed in ethyl acetate extracts and fractions as indicated by their ascorbic acid equivalents.