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Preparation of nalbuphine hydrochloride potentiometric sensors and their applications in pharmaceutical formulations and biological fluids

Maha F.El-Tohamy, Sawsan A.Razeq, Magda Y.El-Maamly, Abdalla A.Shalaby

The construction and performance characteristics of nalbuphine hydrochloride selective electrodes were developed. Three types of electrodes: plastic membrane I, coated wire II, and coated graphite rod III were constructed based on the incorporation of nalbuphine hydrochloride with phosphotungestic acid. The influences of membrane composition, kind of plasticizer, pH of the test solution, soaking time, and foreign ions on the electrodes were investigated. The electrodes showed a Nernstain response with amean calibration graph slope of57.29±0.7, 56.16±0.2 and 55.82±0.9 mV decade-1 at 25ºC for electrode I, II and III respectively, over nalbuphine hydrochloride concentration range from1x10-2-1x10-6M, 1x10-2-5x10-6Mand 5x10-3-5x10-6M, andwith detection limit 4.9x10-7M, 2.4x10-6Mand 2.5 x10-6M for electrode I, II and III respectively. The constructed electrodes gave average selective precise and usable within the pH range 3.5-7.4. Interferences from common cations, alkaloids, sugars, amino acids and drug excipients were reported. The results obtained by the proposed electrodes were also applied successfully to the determination of the drug in pharmaceutical preparations and biological fluids.