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Production of alkaline isoamylase by isolated Micrococcus varians

Iman A.Ghazy, Reda A.Bayoumi, Magdy A.Gad Allah, Samier S.El-Louboudy, Mostafa M.Abo El-Soud

Micrococcus varians isolated fromsoil produced extracellular isoamylase. The optimized conditions for isoamylase production was found when liquid shaking culture was incubated 72h at 30c with initial pH 9.2. The optimum inoculum size on the production of isoamylase was 1000 ml/ 50 ml medium. Soluble starch at 1.5%was the best inducer for enzyme production as a carbon source, and ammonium sulfate at 1%was better than the other nitrogen sources for production of enzyme.