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Profiling of cellular and secreted proteins of selected human pathogenic fungi for selection of species specific diagnostic biomarkers

Jose Scaria, Vandana Nambiar, Pavithra Prabhakar, Thangavelu Thayumanavan, Subramanian Babu

Isolates of Aspergillus niger, A. flavus and Penicillium sp. were recovered fromblood and body fluid samples obtained fromhospital. Extraction procedures were optimized for high degree of recovery of cellular and secreted proteins from the fungal species under study. Single dimension profiling of the proteinswas performed by SDS-PAGE analysis to compare unique as well as differentially expressed proteins among the species. Among the differentially expressed cellular proteins, 28, 39 and 95 kDa specific to A. niger, 70 kDa protein of A. flavus, 27, 37 and 85 kDa proteins of Penicillium sp. were found to showprominent difference in expression. Although many proteins showed difference in the comparison of extracellular profile of the fungal species, the 25 kDa protein of A. flavus was unique to the species. Proteins of 37 and 46 kDa of Penicillium sp. expressed at a higher degree compared to other fungal species. Potential candidate biomarkers can be further characterized by analyzing the expression of the reported proteins in many isolates of these pathogens.