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RAPD analysis of phylogenetic relationship(s) and genetic variation(s) in the genus Allium

Ganesh G.Adsul, Akhilesh K.Chaurasia, Anil V.Dhake, Ramanlal M.Kothari

This mini-review has highlighted the importance of onions as vegetable and has shown its taxonomic position among 750 species of genus Allium. It has compared merits of RandomAmplified Polymorphic DNA (RAPD) markers vs Restriction Fragment Length Polymorphism(RFLP) and established the superiority of RAPD over RFLP. Further, significance of RAPD markers in onion breeding programis indicated and subsequently validated by its multi-faceted applications to (i) assess in-bred integrity, (ii) establish genetic relationship in conjunctionwithmorphologicalmarkers, (iii) prove hybrid status in conjunctionwith genomic in-situ hybridization (GISH), (iv) established origin and relationship between two taxa, (v) establish phylogenetic relationship, (vi) confirminter-specific hybrids, (vii) assess genetic diversity among the onion cultivars and (viii) prepare genetic map of onion cultivars.