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Real Time based RT-PCR Detection of DUF538 Gene Expression in Drought-Challenged Celosia

Ashraf G

To enhance our information in plant system of DUF538 (domain of unknown function 538) protein superfamily, our efforts were made to unravel the relative expression patterns of Celosia DUF538 transcript using real time reverse transcription and polymerase chain reaction (RT-PCR) with template materials collected from the leaves of test plant grown under drought stress condition. The results indicated that DUF538 transcript is highly detectable in drought-challenged leaf tissues. In comparison with non-stressed tissues, the expression level of DUF538 gene was detected about 3.2 - 3.6 folds increase in stressed leaves. The results suggested that DUF538 gene activity may be as one of the drought stress defense responses (possibly though the hydrolytic mechanism) in plant system.