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Simultaneous determination of amlodipine besylate, olmesartan medoxomil and chlorthalidone in pharmaceutical preparations using validated, LCM compatible RP-HPLC method

Janhavi P.Vartak, Shikha M.N.Roy

A simple, fast and precise LCMS compatible reversed phase high performance liquid chromatographic method is developed for the simultaneous determination of Amlodipine Besylate, Olmesartan Medoxomil, Chlorthalidone in pharmaceutical preparations. Chromatographic separation of the three drugs was performed on aMerck Chromolith 4.6 X 50 mm, RP-18e as stationary phase with a mobile phase comprising of Buffer and Methanol in gradient system. Buffer was 0.1%v/v Formic acid (1ml Formic acid to 1000ml water.), filtered and degassed. The chromatographic system was run at a flow rate of 0.6 mLmin-1 and UV detection at 265nm.Method developed was LCMS compatible and the same was used to identify each drug. The proposedmethod was validated for linearity, accuracy, precision, LOD, LOQ and can be conveniently adopted for routine quality control and pharmacokinetic analysis.