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Simultaneous determination of cetrizine and ambroxol in formulation by RP-HPLC

Lakshmi Sivasubramanian, V.Niraimathi

A simple, precise and rapid reversed phase HPLC method was developed for the simultaneous estimation of cetrizine(CT) and ambroxol(AM) in combined formulation. Themethodwas carried out on aHypersilBDS C18 column using a mixture ofwater:methanol (pH adjusted to 3.5 with orthophosphoric acid): tetrahydrofuran(60:37:3 v/v/v) and detection was carried out at 230 nm. The drug CT showed linearity in the range of 4-32mcg/ ml and AM in the range of 48-384mcg/ml. Limits of quantification was found to be 20 and 4ng/ml for CT andAM respectively.