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Simultaneous determination of paracetamol and ibuprofen from combined dosage formulation by HPTLC method

R.V.Rele, S.A.Sawant

In the present communication simultaneous determination of paracetamol and ibuprofen from pharmaceutical preparation by high performance thin layer chromatographic is described. The assay was carried out by using CAMAG Linomat IV applicator in precoated HPTLC plate with silica gel 60F, 20 x10 cm,Merck no. 5642. Ethyl acetate: acetone: butanol : ammonia in the ratio of 30: 40: 30: 10 (v/v) was constituted as solvent system. Diloxanide furoate was used as internal standard. The evaluation was performed at 254 nm. The Rf values of paracetamol and ibuprofen were 0.86 and 0.32 respectively. The calibration curves were found to be linear in the concentration range of 1.2- 6.0 and 1.3-6.5 ìg/ ml for paracetamol and ibuprofen respectively. The results of the analytical methods were validated statistically. The proposed method can be successfully used to determine the drug contents in combined formulation.