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Simultaneous estimation of anti-hypertensive drugs and a vitamin in a pharmaceutical formulation

Avdhoot J.Laud, Suhas Pednekar, Vikas V.Vaidya, Santosh S.Joshi, Sagar A.Parekh

A simple, rapid, and precise reversed-phase high-performance liquid chromatographic method has been developed for simultaneous determination of Hydrochlorothiazide; Folic Acid; Atenolol; Aspirin; Enalapril Maleate and Atorvastatin Calcium. The six drugs were separated on an Inertsil; 150mm; 4.6mm; i.d. 5µm particle, C8 column. The mobile phase was buffer: methanol with a binary gradient program at a flow rate of 2.0mL min-1. The bufferwas amixture of 0.01MSodiumDi-hydrogenOrtho-Phosphate (NaH2PO4); 0.01M of Di-Sodium Hydrogen Ortho-Phosphate (Na2HPO4) and 1.0 g of Sodium-Heptane Sulphonic acid in 1000mL purified water and pH adjusted to 2.6 with ortho-phosphoric acid. UV detection was performed at 215nm. The method was validated for linearity, accuracy, and precision parameters and these parameters were acceptable in the ranges 33.043µg mL-1 to 99.129µg mL-1 for Hydrochlorothiazide, 3.659µg mL-1 to 10.977µg mL-1 for folic acid, and 126.000µg mL-1 to 378.000µg mL-1 for Atenolol, 374.000µg mL-1 to 1122.000µg mL-1 for Aspirin, 10.000µg mL-1 to 40.000µg mL-1 for Enalapril, and 49.811µg mL-1 to 149.433µg mL-1 for Atorvastatin.