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Simultaneous RPHPLCdetermination of camylofin dihydrochloride and diclofenac potassiumin pharmaceutical preparations

M.V.Rathnam, R.R.Singh, R.Vegesna

A simple, fast and precise reversed phase high performance liquid chromatographic method is developed for the simultaneous determination of camylofin dihydrochloride and diclofenac potassiumusingmethyl paraben as an internal standard. Chromatographic separation of these two drugs was performed on inertsil C18 column (250mmï‚´ 4.6mm, 5 µm) as stationary phasewith amobile phase comprising of 0.05MKH2PO4 :methanol (35:65) at a flowrate of 1.5mLmin-1 andUVdetection at 220 nm. The Retention time of Methyl paraben, Camylofin dihydrochloride and diclofenac potassium were 3.60 min, 4.85 min and 13.10 min respectively. The proposed method was validated for linearity, accuracy, precision, LOD, LOQ. Linearity, accuracy and precision were found to be acceptable over the ranges of 250-750 - µg mL-1 for both camylofin dihydrochloride and diclofenac potassium. It can be conveniently adopted for routine quality control analysis.