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Sodium Dodecyl Sulphate as a Surfactant Medium for Photometric and Fluorometric Sensing of Nickel (II) Using 1-(2-Hydroxyphenyl) Thiourea

A. Sunil and S. Jagadeeswara Rao

The determination of nickel (II) was achieved using 1-(2-hydroxyphenyl) thiourea (OHPTU) reaction system under neutral pH conditions at pH of 7.0 using phosphate buffer with spectrophotometry and spectrofluorimetry. The entire study was done at normal laboratory temperature, 25oC. The activator system that suited for the current study was 1% pyridine and the surfactant system was 1% SDS. The synergy of both pyridine and SDS led to the enhancement of sensitivity. Using the current methods, nickel (II) was determined in the range 0.6 ng/mL – 100 ng/mL. Stern-Volmer relation for fluorescence quenching was obeyed. The method was successfully applied to determination of nickel (II) in plant and waste water samples.