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Spectrophotometric andHPLCmethods for simultaneous estimation of ramipril and hydrochlorothiazide fromcombined dosage forms

GopalGarg, Shailendra Saraf, Swarnlata Saraf

Two simple, accurate, economical and reproducibleUVspectrophotometric and one HPLC method for simultaneous estimation of two-component drugmixture of ramipril and hydrochlorothiazide in combined tablet dosage form have been developed. The first developed method employs simultaneous equation method using mixed standards and 216.5nm and 273.0nm as wavelength maxima for estimation. The second method involves first derivative spectroscopy using 221.5nm and 283.0nm as zero crossing points for hydrochlorothiazide and ramipril respectively. For both spectrophotometric methods, 0.1N sodium hydroxide was used as solvent. DevelopedHPLCmethod is reverse-phase chromatographicmethod using C18 column and methanol: water in ratio of 90:10 asmobile phase, at a flow rate 1.3ml/min. The separation was monitored by UV detection at 214nm. Paracetamol was used as internal standard forHPLCmethod. Linearity was observed in concentration range of 2-14g/ml of ramipril and hydrochlorothiazide. Results of analysis were validated statistically and by recovery studies.