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Spectroscopic determination of metformin hydrochloride by using ninhydrin

S.B.Virupaxappa, K.H.Shivaprasad, M.S.Latha, G.A.Swetha

A simple and sensitive spectrophotometric method has been developed and validated for the estimation of metformin hydrochloride in bulk and in tablet formulation. The primary amino group of metformin hydrochloride reactswith ninhydrin in alkalinemediumto forma violet colour chromogen, which is determined spectrophotometrically at 572 nm. It obeyed BeerÂ’s law in the range of 9-19 µg/ml. Percentage recovery of the drug for the proposed method ranged from 97-100% indicating no interference of the tablet excipients. The proposed method was found to be accurate and precise for routine estimation ofmetformin hydrochloride in bulk and fromtablet dosage forms.