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Stability indicating methods for determination of nafronyl oxalate

Samah S.Abbas, Hala E.Zaazaa, Mohamed R.El-Ghobashy, Yasmin M.Fayez, Soheir A.Fattah

HPLC method with spectroscopic detection for determination of Nafronyl (Naftidrofury)lOxalate (NFL), a rapid separation could be achieved by a C18 column using mobile phases of 72%methanol-1% triethylamine -0.6% phosphoric acid. The eluate was monitored at 282.4. Two chemometric techniques were applied for determination of NFL namely partial least squares (PLS-1) and genetic algorithm based wavelength selection- partial least squares (GA-PLS) regression methods. The suggested methods were used to determine NFL in syntheticmixtures and in commercial tablets. The validity of the proposed methods was further assessed by applying standard addition technique. The obtained results were statistically compared with official method, showing no significant difference with respect to accuracy and precision.