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Strategies for LC-MS Development in Quantitative Bioanalysis

Kelvin James

Although liquid chromatography and atmospheric pressure ionisation tandem mass spectrometry (LC-MS/MS) have been used for quantitative bioanalysis for over fifteen years, new ideas and technologies are always being developed to improve the several processes of quantitative LC-MS/MS bioanalysis. To further advance biological sample collection, storage, and extraction, chromatography, and mass spectrometric detection, we have focused on concepts and technologies that have just recently been developed. Under these major headings, a number of specific topics are presented, summarising the most recent findings in these fields. Off-line and on-line plasma extraction, enhanced mass resolution, atmospheric pressure photoionization, high-field asymmetric waveform ion mobility spectrometry, electron capture atmospheric pressure chemical ionisation, improving MS detection via formation of anionic and cationic adducts, and chemical derivatization, ultra-performance chromatography, hydrophilic interaction chromatography, and MS-friendly ion-pair resolution are some of the topics covered. In our final section, we go through possible hazards in LC-MS/MS bioanalysis and how to prevent them. These traps can be caused by the matrix effect, which is a wild card phenomena, the use of improper calibration standards and quality control samples for analyses involving unstable medicines or metabolites, and mass spectrum interference from metabolites or prodrugs.