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Study of protein expression in Pseudomonas putida and Pseudomonas aeruginosa on degarding hydrocarbon compounds (Diesel)

A.Xavier Vergeese Raja, Hiranmai Yadav, S.Anil Kumar, N.Venkata Lakshmi, A.S.A.Mohamed Riswana

Bioremediation is the process of using agents to convert or degrade organic compounds such as crude oil and gasoline in to non-toxic substances such as carbon dioxide and water. Oil spill in the environment is a problem for which bio remediation by micro organisms is a natural, viable and economic solution. When an oil spill occurs often due to a ruptured oil tanker or leaking oilrig, people around the world are awed by the damage to the environment. However, there are also naturally occurring oil spills such as sea page from oil deposits beneath ocean floor. Regardless of their origins oil spills have an impact on the environment. Bacteria capable of degrading oil includes Gram negative rods such as Pseudomonas, Acetobacter, FlavoBacteria, Cornibacterium and Alcaligenes. The hydrocarbon (Diesel) degrading strains are separated from the nutrient broth medium and proteins are extracted and compared with the proteins extracted from the strains grown without adding hydrocarbon compound. The total protein concentration in the treated Pseudomonas putida and Pseudomonas aeruginosa was 55g and 35g respectively whereas the concentration in the normal Pseudomonas putida and Pseudomonas aeruginosa was found to be 30g and 25g.