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Validated capillary electrophoresis method the quantification of sodium metabisulfite in cephalosporin using indirect UV detection

Gajanan B.Kasawar, Mazahar N.Farooqui

The capillary electrophoresis (CE) method for the determination of sodium metasbisulfite content in cephalosporin drugs was developed using potassium hydrogen phthalate (5mM) and cetyl trimethyl ammonium bromide (0.25mM) as a flow modifier at pH 7.2 along with the application of an electric field of 20kV in 0.4 minute, using a 40 cm a fused silica capillary. Indirect UV detection was performed at a wavelength of 210 nm. Linearity and accuracywere performed in the concentration range of 4-24gmL-1 and 1-2.4g mL-1 respectively. The method was validated to show specificity, linearity, accuracy, precision, ruggedness, robustness and stability in analytical solution. The limit of quantification and detection were 1.673g mL-1 and 1.402gmL-1 respectively.